Seroprevalence of infection with Trypanosoma cruzi and associated factors in a population of Cumaral, Meta, Colombia
Keywords:
Trypanosoma cruzy, seroeprevalence, ELISA, Chagas disease, Meta, Colombia, parasitic zoonosis, associated factors.Abstract
Introduction: Chagas disease is a parasitic zoonosis caused by Trypanosoma cruzi , a vector-born protozoan that is transmitted to humans. Field studies predict that one-third of around 18 million T cruzi-infected humans will die of Chagas disease in Latin America.
Objective: To determine the seroprevalence of the infection with Trypanosoma cruzi and to identify some risk factors associated with this condition in Cumaral, Meta, Colombia.
Material and Methods: A descriptive, cross-sectional study was conducted. Blood samples were collected from subjects that had been living in urban and rural areas of Cumaral municipality for more than six months. An ELISA-IgG method with total extracts (first test) was used for the detection of Trypanosoma cruzy IgG. A recombinant-based ELISA was performed as a confirmatory test. Doubtful results were sent to the National Institute for Health for confirmation by indirect immunofluorescence assay. Prism 8 for MacOS (version 8.2.1) was used for statistical analysis.
Results: In the screening for Chagas disease, 2,7 % of all the cases tested were positive. When comparing subjects with positive and negative results, differences between age, scholarship and materials used in the construction of house walls were evidenced.
Conclusions: The results provide useful information about housing conditions and seroprevalence of the disease that help to evaluate the effectiveness of the arrangement that provides and strengthens the access to the diagnosis and treatment of the disease.Downloads
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