Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion
Keywords:
Threat of abortion, genetic polymorphism, human leukocytic antigens.Abstract
Introduction: The polymorphism of the human leukocyte antigen G gene is investigated in different complications related to pregnancy; however, it must be verified if there is an association between this genetic polymorphism and the threat of abortion.
Objective: To establish the genetic association between the 14 base pair insertion/deletion polymorphism in exon 8 of the three non-translatable prime region of the human leukocyte antigen G gene with susceptibility to the threat of abortion in pregnant women from Camagüey.
Material and Methods: A case-control study was carried out, involving 22 pregnant women with threatened abortion and 65 pregnant women (control group). For the study, human deoxyribonucleic acid was obtained from peripheral blood, the polymerase chain reaction was used for the molecular typing of the genotypes and alleles that were identified by agarose gel electrophoresis.
Results: The most frequent genotype in the group of pregnant women with threatened abortion was insertion/deletion, while in the control group the deletion/deletion genotype predominated.
Conclusions: The insertion/deletion polymorphism of 14 base pairs in exon 8 of the 3’ non-translatable region of the human leukocyte antigen G gene represents a risk factor for the appearance of threatened abortion in pregnant women from Camagüey, while the expression of the deletion/deletion genotype represents a protective factor against this health condition.Downloads
References
1. Botelho SM. Avaliação das moléculas HLA-G e PD-L1 em gestantes submetidos a transplante renal em Goiânia-GO [Tesis]. Goiânia: Universidad Federal de Goiás; 2020 [Citado 25/07/2023]. Disponible en: https://repositorio.bc.ufg.br/tede/bitstream/tede/11702/3/Tese%20-%20Silvia%20Mar%c3%a7al%20Botelho%20-%202020.pdf
2. Vaquero-Yuste C, Juárez I, Molina-Alejandre M, Molanes-López EM, López-Nares A, Suarez-Trujillo F, et al. HLA-G 3’UTR Polymorphisms Are Linked to Susceptibility and Survival in Spanish Gastric Adenocarcinoma Patients. Front Immunol [Internet]. 2021 [Citado 25/07/2023];12:698438. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453083/
3. Enríquez de Salamanca Carrascosa M. Estudio del polimorfismo del gen inmunosupresor HLA-G, en amerindios [Tesis doctoral]. Madrid: Universidad Complutense de Madrid; 2018 [Citado 25/07/2023]. Disponible en: https://docta.ucm.es/rest/api/core/bitstreams/6346db9b-417d-42ae-93c5-09ed5c8a2d77/content
4. Nilsson LL, Scheike T, Langkilde CH, Jørgensen N, Hornstrup MB, Perin TL, et al. Examining extended human leukocyte antigen-G and HLA-F haplotypes: the HLA-G UTR-4 haplotype is associated with shorter time to pregnancy in an infertility treatment setting when both female and male partners are carriers. Fertility and Sterility [Internet]. 2020 Sep [Citado 25/07/2023];114(3):628-39. Disponible en: https://www.sciencedirect.com/science/article/pii/S0015028220304179?ref=pdf_download&fr=RR-2&rr=80179aeb1d62221a
5. Emadi E, Akhoundi F, Kalantar SM, Emadi-Baygi M. Predicting the most deleterious missense nsSNPs of the protein isoforms of the human HLA-G gene and in silico evaluation of their structural and functional consequences. BMC Genetics [Internet]. 2020 Ago [Citado 25/07/2023];21:94. Disponible en: https://bmcgenomdata.biomedcentral.com/articles/10.1186/s12863-020-00890-y
6. Rouas-Freiss N, Moreau P, LeMaoult J, Papp B, Tronik-Le Roux D, Carosella ED. Role of the HLA-G immune checkpoint molecule in pregnancy. Hum Immunol [Internet]. 2021 May [Citado 10/08/2023];82(5):353-61. Disponible en: https://pubmed.ncbi.nlm.nih.gov/33745758/
7. Merdas AH, Iqbal MN, Alyasiri NS. The Effects of HLA-G Gene Polymorphism and sHLA-G Level in Women with Threatened Abortion. J Techniques [Internet]. 2022 [Citado 10/08/2023];4(2):69-74. Disponible en: https://journal.mtu.edu.iq/index.php/MTU/article/view/505
8. Quirós Rodríguez Y, Arrieta García R, Prado Téllez NI, Pimentel Benítez HI, Amador Aguilar LM. Caracterización clínico epidemiológica de las defunciones fetales de causa genética en Camagüey. Rev Cubana Med Gen Integr [Internet]. 2022 Sep [Citado 29/11/2023];38(3):e1820. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-21252022000300005&lng=es. Epub 01-Sep-2022.
9. Lardoeyt Ferrrer R. Fundamentos de genética médica poblacional [Internet]. La Habana: Editorial Ciencias Médicas; 2016 [Citado 27/05/2024]. Disponible en: www.ecimed.sld.cu/2016/11/17/fundamentos-de-genetica-medica-poblacional
10. Promega. Isolation of Genomic DNA from Whole Blood [Internet]. USA: Promega Corporation; 2020 [Citado 10/08/2023]. Disponible en: https://worldwide.promega.com/-/media/files/resources/protcards/wizard-genomic-dna-purification-kit-quick-protocol.pdf
11. Rizzo R, Hviid TVF, Govoni M, Padovan M, Rubini M, Melchiorri L, et al. HLA-G genotype and HLA-G expression in systemic lupus erythematosus: HLA-G as a putative susceptibility gene in systemic lupus erythematosus. Tissue Antigens [Internet]. 2008 [Citado 03/05/2024];71(6):520-9. Disponible en: https://doi.org/10.1111/j.1399-0039.2008.01037.x
12. Manzini JL. Declaración de Helsinki: Principios éticos para la investigación médica sobre sujetos humanos. Análisis de la 5ta reforma, aprobada por la Asamblea General de la Asociación Medica Mundial en octubre del año 2000, en Edimburgo, respecto del texto aprobado en Somerset West (Sudáfrica) en octubre de 1996. Acta bioeth [Internet]. 2000 Dic [Citado 10/08/2023];6(2). Disponible en: https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S1726-569X2000000200010
13. Krop J, Van Der Keur C, Kapsenberg JM, Den Hollander F, Van Der Hoorn MLP, Heidt S, et al. Soluble HLA-G blood levels are not increased during ongoing pregnancy in women with a history of recurrent pregnancy loss. J Reprod Immunol [Internet]. 2022 Sep [Citado 10/08/2023];153:103665. Disponible en: https://www.sciencedirect.com/science/article/pii/S0165037822001942
14. Monti M, Lupoli R, Fernández LMS, Cirillo F, Di Minno MND. Association of human leukocyte antigen-G 14 bp polymorphism with recurrent pregnancy loss in European countries: a meta-analysis of literature studies. Fertil Steril [Internet]. 2019 Sep [Citado 25/07/2023];112(3): 577-85. Disponible en: https://pubmed.ncbi.nlm.nih.gov/31280950/
15. Sipak O, Rył A, Grzywacz A, Laszczyńska M, Szymański S, Karakiewicz B, et al. Molecular Analysis of HLA-G in Women with High-Risk Pregnancy and Their Partners with Regard to Possible Complications. Int J Environ Res Public Health [Internet]. 2019 Mar [Citado 25/08/2023];16(6):982. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466015/
16. Sipak O, Rył A, Grzywacz A, Laszczyńska M, Zimny M, Karakiewicz B, et al. The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy. Int J Environ Res Public Health [Internet]. 2019 May [Citado 10/08/2023];16(9):1546. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539819/
17. Marik B, Nomani K, Agarwal N, Dadhwal V, Sharma A. Role of the HLA‐G regulatory region polymorphisms in idiopathic recurrent spontaneous abortions (RSA). Am J Reprod Immunol [Internet]. 2023 [Citado 10/08/2023];90(2):e13740. Disponible en: https://pubmed.ncbi.nlm.nih.gov/37491923/
18. Bai W, Lin Z, Ye J, Zhou L, Xi J, Cai W. Frequency of HLA‑G UTR‑1/UTR‑3/UTR‑7 in women with unexplained recurrent spontaneous abortion. ExpTher Med [Internet]. 2022 [Citado 25/08/2023];24(6):1-8. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634346/
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