Evaluation of the antifungal activity of ketoconazole against strains of Microsporum sp. and development of a pharmaceutical cream formulation

Authors

Keywords:

ketoconazole cream, topical antifungal formulation, dermatophyte infections, microbial, Microsporum sp.

Abstract

Introduction: In Ecuador, the prevalence of dermatophytes is influenced by various factors such as environmental conditions, sociocultural practices, occupational exposures, hygiene routines and individual immune status; however, despite the significant burden of fungal diseases, there is a lack of data in Ecuador, which hinders the development of effective control and monitoring strategies.

Objective: To evaluate the antifungal activity of ketoconazole against M. canis strains and develop a pharmaceutical cream formulation to treat infections caused by this dermatophyte.

Results: Both formulations exhibited satisfactory physical stability. However, only formulation A containing EDTA demonstrated effective inhibition of microbial growth, as evidenced by the absence of pink discoloration and bubble formation. In contrast, formulation B showed signs of microbial contamination, probably due to an inadequate preservative content. Additionally, clinical studies demonstrated the effectiveness of ketoconazole 2% cream in the treatment of various dermatophyte infections, comparable to clotrimazole cream.

Conclusions: MIC results varied between studies and even between strains of the same species. Using the MIC breakpoints for Candida albicans, the obtained result of 4 μg/mL indicates that M. canis shows intermediate sensitivity to the drug.

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Published

2024-07-31

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1.
Basantes Vaca CV, Parra Álvarez PF, Serrano Torres GJ, Torres Rodríguez SH, Navarrete Reino LM. Evaluation of the antifungal activity of ketoconazole against strains of Microsporum sp. and development of a pharmaceutical cream formulation. Rev haban cienc méd [Internet]. 2024 Jul. 31 [cited 2025 Jul. 6];23:e5752. Available from: https://revhabanera.sld.cu/index.php/rhab/article/view/5752

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