Influence of glycerin on the color stability of a compound resin: an in vitro study
Keywords:
Composite resins, glycerine, color stability, aesthetic dentistryAbstract
Introduction: Chromatic stability of compound resins determines their aesthetic success and clinical longevity. The oxygen-inhibited layer formed during photopolymerization promotes surface staining and color change. Glycerin has been proposed as a barrier for optimizing curing and preserving color.
Objective: To determine the influence of glycerin application on the color stability of a compound resin.
Material and Methods: An in vitro, cross-sectional, quantitative experimental study was conducted. The sample included: 30 discs of Filtek Z350 resin (3M) randomly assigned to two groups (n=15): Group A without glycerin and Group B with glycerin applied in the final increment. Color evaluation was performed using a Runyes 3DS intraoral scanner, under standardized conditions. Statistical analysis was conducted using SPSS v22.
Results: Samples with glycerin showed lower color change compared to those without glycerin. Comparison between groups revealed statistically significant differences (p = 0.032). Glycerin acted as an oxygen barrier, reducing the formation of the inhibited layer and promoting more complete superficial polymerization, with a direct impact on color preservation.
Conclusions: Applying glycerin during photopolymerization significantly improves the color stability of composite resins and represents a simple technique of high clinical value to optimize the aesthetics and durability of restorations. Its incorporation into restorative protocols is recommended, as well as conducting in vivo studies to confirm these findings under real clinical conditions.
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1. Dávila-Ventura S, Roncal-Espinoza RJ. Conocimiento sobre carillas dentales en estudiantes universitarios peruanos. Rev Fac Odontol Univ Antioquia [Internet]. 2023;35(1):36–46. Disponible en: http://doi.org/10.17533/udea.rfo.v35n1a3
2. Loarte Merino GJ. Fundamentos para elegir una resina dental. Odontol Act Rev Científica [Internet]. 2019;4(Esp):57–64. Disponible en: http://doi.org/10.31984/oactiva.v4iEsp.408
3. Aquino Valverde AJ, Aguilar Vargas G del P, Díaz Fernández JM, Leiva Ramírez PA, Quintanilla Labajos DA, Atoche Socola KJ, et al. Efectividad de fotopolimerización usando lámparas led: una revisión. Rev Científica Odontológica [Internet]. 2022;10(3):e120. Disponible en: http://doi.org/10.21142/2523-2754-1003-2022-120
4. Ramírez Fernández L, Colán Guzmán P del R, Valencia Heredia JJ, Guevara Canales JO, Morales Vadillo R, Ramírez Fernández L, et al. ¿La glicerina influye en la estabilidad del color de la resina compuesta? Rev Cuba Estomatol [Internet]. 1995; 2022 [Citado 17/10/2025];59(2). Disponible en: http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S0034-75072022000200002&lng=es&nrm=iso&tlng=es
5. Pacheco ETJ, López MAC. Fotopolimerización en odontología. Braz J Health Rev [Internet]. 2024;7(1):4210–20. Disponible en: http://doi.org/10.34119/bjhrv7n1-342
6. ISO. Dentistry: Polimer-based restorative materials ISO 4049:2019 [Internet]. EE.UU: International Standard confirmed; 2019. [Citado 17/10/2025]. Disponible en: https://www.iso.org/standard/67596.html
7. Ciocan LT, Biru EI, Vasilescu VG, Ghitman J, Stefan A-R, Iovu H, et al. Influence of Air-Barrier and Curing Light Distance on Conversion and Micro-Hardness of Dental Polymeric Materials. Polymers. Multidisciplinary Digital Publishing Institute [Internet]. 2022;14(24):5346. Disponible en: http://doi.org/10.3390/polym14245346
8. Soares L de M, Romano B de C, André CB, Giannini M. Effect of glycerin gel application for post-curing process on the flexural strength, elastic modulus, and microhardness of 3D printing resins. Braz Dent J [Internet]. 2025;36:e23. Disponible en: https://doi.org/10.1590/0103-644020256361
9. Güler AU, Güler E, Yücel AC, Ertaş E. Effects of polishing procedures on color stability of composite resins. J Appl Oral Sci Rev FOB [Internet]. 2009;17(2):108–12. Disponible en: http://doi.org/10.1590/s1678-77572009000200007
10. Aydın N, Topçu F-T, Karaoğlanoğlu S, Oktay E-A, Erdemir U. Effect of finishing and polishing systems on the surface roughness and color change of composite resins. J Clin Exp Dent [Internet]. 2021;13(5):e446–54. Disponible en: http://doi.org/10.4317/jced.58011
11. Uctasli MB, Garoushi S, Uctasli M, Vallittu PK, Lassila L. A comparative assessment of color stability among various commercial resin composites. BMC Oral Health [Internet]. 2023;23(1):789. Disponible en: http://doi.org/10.1186/s12903-023-03515-9
12. Ohno Y. CIE fundamentals for color measurements. In: NIP & Digital Fabrication Conference [Internet]. Maryland: Society of Imaging Science and Technology; 2000.
13. Schanda J, International Commission on Illumination, eds. Colorimetry: understanding the CIE system [Internet]. Austria: CIE/Commission internationale de l’eclairage Wiley-Interscience; 2007.
14. Islam MS, Thahab TS, Alhayally AT, Padmanabhan V, Aryal ACS, Rahman MM. The Influence of Different Factors on Shade-Taking Accuracy Using Digital Shade Guide. Open Dentristry J [Internet]. 2024;18(1). Disponible en: http://doi.org/10.2174/0118742106315626240722093623
15. Ahmed YT, Almutairi FA, Alomran SA, Alkhayatt NM, Alsulaiman SA, Alohali SY, et al. Dehydration Time Effect on Tooth Color Measurement: An In Vitro Study. Eur J Dent [Internet]. 2022;16(4):895–900. Disponible en: http://doi.org/10.1055/s-0041-1741377
16. Khazaal G, Daou M, Mahdi S-S, Ahmed Z, Maalouf E, Batteni G, et al. In vitro evaluation of the color stability and surface roughness of a new composite flow. J Clin Exp Dent [Internet]. 2023;15(1):e43–50. Disponible en: http://doi.org/10.4317/jced.60005
17. El-Rashidy AA, Abdelraouf RM, Habib NA. Effect of two artificial aging protocols on color and gloss of single-shade versus multi-shade resin composites. BMC Oral Health [Internet]. 2022;22(1):321. Disponible en: http://doi.org/10.1186/s12903-022-02351-7
18. Jablonski T, Takahashi MK, Brum RT, Rached RN, Souza EM. Comparative study of the fluorescence intensity of dental composites and human teeth submitted to artificial aging. Gen Dent. 2014;62(1):37–41.
19. Strnad G, Kovacs M, Andras E, Beresescu L. Effect of Curing, Finishing and Polishing Techniques on Microhardness of Composite Restorative Materials. Procedia Technol [Internet]. 2015;19:233–8. Disponible en: http://doi.org/10.1016/j.protcy.2015.02.034
20. Borges MG, Silva GR, Neves FT, Soares CJ, Faria-E-Silva AL, Carvalho RF, et al. Oxygen Inhibition of Surface Composites and Its Correlation with Degree of Conversion and Color Stability. Braz Dent J [Internet]. 2021;32(1):91–7. Disponible en: http://doi.org/10.1590/0103-6440202103641
21. Robertson L, Phaneuf M, Haimeur A, Pesun I, França R. Degree of Conversion and Oxygen-Inhibited Layer Effect of Three Dental Adhesives. Dent J Multidisciplinary Digital Publishing Institute [Internet]. 2016;4(4):37. Disponible en: http://doi.org/10.3390/dj4040037
22. Lee Y-K, Yu B, Lim H-N, Lim Ji. Difference in the color stability of direct and indirect resin composites. J Appl Oral Sci [Internet]. 2011;19(2):154–60. Disponible en: http://doi.org/10.1590/S1678-77572011000200012
23. Servian L. Importancia del acabado y pulido en restauraciones con resinas compuestas en dientes anteriores. Reporte de caso clínico. Rev Científica Cienc Salud [Internet]. 2019 [Citado 17/10/2025];1(1):52–6. Disponible en: https://dialnet.unirioja.es/servlet/articulo?codigo=10009738
24. Francisco A, Dey T, Mendes JM, Sousa A, Torres O. Influence of Polishing Systems on Color Stability of Dental Composite Resins: An In Vitro Study. J Res Dent Maxillofac Sci [Internet]. 2024;9(3):193–203. Disponible en: http://doi.org/10.61186/jrdms.9.3.193
25. Villacis J. influencia del uso de la glicerina en la estabilidad del color de una resina compuesta, estudio in vitro. Perú: Universidad Nacional de Chimborazo; 2025.
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