Vascular Endothelial Growth Factor gene +405G>C polymorphism in the Cuban population
Keywords:
Vascular Endothelial Growth Factor, Population Genetics, Gene Frequency.Abstract
Introduction: The vascular endothelial growth factor (VEGF) is a protein involved in the proliferation and cell migration of the vascular endothelium. In its gene, +405G>C Polymorphism has been reported. There are no population genetic reports of this variant in Cuba that allow the characterization of immunogenetic profiles at a molecular level for its application to allelic association studies.
Objective: To describe the genic and genotypic frequencies of the VEGF (+405 G>C) polymorphism in the Cuban population.
Material and Methods: A descriptive cross-sectional observational study was carried out from October 2017 to March 2018 in 162 Cuban healthy newborns of both sexes for the neonatal screening for metabolic diseases, whose biological samples were conserved in the DNA bank of the National Center for Medical Genetics. The molecular characterization of the genotypes was carried out using a PCR-ARMS. The GENEPOP 4.4 software and the statistical software package STATISTICA 8.0 were used for the analysis of genic and genotypic frequencies.
Results: The population did not adjust to the Hardy-Weinberg equilibrium model for the gene evaluated. The estimated gene frequencies of VEGF +405 G> C polymorphism were 0.33 for the G allele and 0.67 for the C allele. The calculation of the genotypic frequencies resulted in 0.14, 0.37 and 0.49, for the variants GG, GC and CC, respectively.
Conclusions: The allelic frequencies of VEGF.C were higher than the frequencies of the VEGF.G allele, being the VEGF GG the least represented genotype in the group studied.
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References
1. Weizmann Institute of Science. GeneCards. Human Gene Database. [En línea].; 2019 [Citado 20 May 2019]. Disponible en: http://www.genecards.org/cgi-bin/carddisp.pl?gene=VEGFA&keywords=VEGF.
2. Online Mendelian Inheritance in Man. Vascular Endothelial Growth Factor. [En línea].; 2019 [Citado 20 May 2019]. Disponible en:
https://www.omim.org/entry/192240?search=vegf&highlight=vegf.
3. Karaman S, Leppänen VM, Alitalo K. Vascular endothelial growth factor signaling in development and disease. Development. 2018;145(14).
4. Hamedian A, Esteghamati A, Noshad , Mozafari , MoinTavakkoli H, Nakhjavani M, et al. Vascular endothelial growth factor (VEGF) +405 C/G polymorphism is associated with essential hypertension in a population from Tehran of Iran. Mol Biol Rep. 2012 May; 39(5): 6213–6218.
5. Rezaei M, Hashemi M, Sanaei S, Mashhadi MA, Taheri M. Association Between Vascular Endothelial Growth Factor Gene Polymorphisms with Breast Cancer Risk in an Iranian Population. Breast Cancer (Auckl). 2016; 10:85–91.
6. Paradowska-Gorycka A, Pawlik A, Romanowska-Prochnicka K, et al. Relationship between VEGF Gene Polymorphisms and Serum VEGF Protein Levels in Patients with Rheumatoid Arthritis. PLoS One. 2016; 11(8): e0160769.
7. Hajiluian G, Abbasalizad Farhangi M, Jahangiry L. Mediterranean dietary pattern and VEGF +405 G/C gene polymorphisms in patients with metabolic syndrome: An aspect of gene-nutrient interaction. PLoS One. 2017; 12(2): e0171637.
8. Zablotna M, Sobjanek M, Nedoszytko B, Lange M, Kozicka D, Glen J, et al. Association of psoriasis with the VEGF gene polymorphism in the northern Polish population. J Eur Acad Dermatol Venereol. 2011.
9. Banyasz I, Szabo S, Bokodi G, Vannay A, Vasarhelyi B, Szabo A, et al. Genetic polymorphisms of vascular endothelial growth factor in severe pre-eclampsia. Mol Hum Reprod. 2006;(12): 233–6.
10. Perini JA, Cardoso JV, Berardo PT, et al. Role of vascular endothelial growth factor polymorphisms (-2578C>A, -460 T>C, -1154G>A, +405G>C and +936C>T) in endometriosis: a case-control study with Brazilians. BMC Womens Health. 2014; 14:117.
11. Eller A, Branch D, Nelson L, Porter T, Silver R. Vascular endothelial growth factor-A gene polymorphisms in women with recurrent pregnancy loss. J Reprod Immunol. 2011;(88): 48–52.
12. Ghasemi N, Dehghani Firouzabadi R, Ahmadi S. Association of -460C/T and +405 G/C polymorphisms of vascular endothelial growth factor gene and susceptibility to ovarian hyperstimulation syndrome. Int J Reprod Biomed (Yazd). 2017;15(2):87–92.
13. Protocolo Normalizado de Operaciones de extracción de ADN. La Habana: Centro Nacional de Genética Médica, Biología Molecular; 2017.
14. Boudjenah R, Molina-Gomes D, Torre A, Bergere M, Bailly M, Boitrelle F, et al. Genetic Polymorphisms Influence the Ovarian Response to rFSH Stimulation in Patients Undergoing In Vitro Fertilization Programs with ICSI. PlosOne. 2012 Junio; 7(6).
15. Levene H. On a matching problem arising in genetics. Annals of Mathematical Statistics. 1949; 20: 91-94.
16. Marcheco-Teruel B, Parra E, Fuentes-Smith E, Salas A, N. Buttenschøn H, Demontis D, et al. Cuba: Exploring the History of Admixture and the Genetic Basis of Pigmentation Using Autosomal and Uniparental Markers. PLoS Genet. 2014; 10(7): e1004488.
17. dbSNP Short Genetic Variations. [En línea].; 2018 [Citado 25 Jul 2018]. Disponible en: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=2010963.
18. dbSNP Short Genetic Variations. [En línea].; 2018 [Citado 25 Jul 2018]. Disponible en: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_viewTable.cgi?pop=1303.
19. Lardoeyt Ferrer R. Ley de Hardy Weinberg. En: Lardoeyt Ferrer R, Taboada Lugo N, Vázquez Sánchez V, Marcheco Teruel B, Rojas Betancourt I, Herrera Martínez M, et al. Fundamentos de genética médica poblacional. La Habana: Editorial Ciencias Médicas; 2016: 20-30.
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